【争鸣】从YEAST全蛋白质组鉴定看质谱仪器和技术飞速发展


2001, Yates, MudPIT,Sequest, 68hrs, 1483 proteins (0.4 protein/min) . Nat Biotech,2001, 19, 242-247 

2002, Gygi, offline fractionation, LC/LC.MS/MS,  135hrs, 1504 proteins (0.2 protein/min).  JPR,2012 2, 43-50 

2003, Weissmann,  genome to predict 4500 proteins【Yeast , 6600 ORF】

2006, Mann, LTQ-Orbitrap, SILAC, 48hrs, 2003 proteins (0.7 protein/min). Genome Biol. 2006; 7(6): R50

2008, Mann, LTQ-Orbitrap, SILAC , fractionation, triplicate aquiring, 144hrs, 4000 proteins (0.5 protein/min).  Nature 455, 1251-1254. Genome Biol. 7(6): R50 

2010, Coon, fractionation, multipile proteases (trypsin, LysC, ArgC, AspN, and GluC), 320hrs,3908 proteins (0.2 protein/min). JPR, 2010, 9, 1323-1329 

2011, Muddiman, 5600 @low speed 20Hz for MS2, 1112 proteins [ Although the TripleTOF is capable of operating at very high scan rates (up to 100 Hz), 20 MS/MS events provided a greater number of protein identifications for this global analysis]. Analytical Chemistry 83, 5442-5446 【此结果存疑，待核对更多数据】

2011,Yasushi Ishihamaa, 5600，4ug HeLa cell,4mX100um i.d. monolithic silica-C18【4米长硅胶整体柱】,8h gradient,500 nL/min,41319 nrTryptic peptides,5,970 proteins. J. Chromatogr. A (2011), doi:10.1016/j.chroma.2011.10.059 

2012，Aebersold，DIA and TDE. SWATH【32X25Da precursor】.dynamic range of 4 orders.MCP,2012,11(16):1?7

2012, Mann, Single-shot Ultra HPLC, QE, 4hrs, 3900 proteins (16 protein/min). 33 fold. MCP,2012,11(3):M111.013722

2013, Coon,Fusion. Trypsin+DMSO. MS:m/z300-1500, Res@60K@m/z200, MS/MS:20Hz, Q-isolation 0.7Th(FWHM)(unit mass resolution), 5 second/cycle, 1.4ug, 1.5hr run (70min gradient), 3977 proteins (44 protein/min). Dynamic range:5000. PRM comparable to MRM【未提供详细比对结果，待验证】

【上述数据全部来自老外的文献，国内做的结果未纳入，因此，上述fact肯定不全面】

【wkh, 2014-04-22 13:26:22】 【责任人 wkh】 [已阅读 6962 次]