Native Mass Spectrometry-Towards High-Throughput Structural Proteomics

Native mass spectrometry (MS) has become a sensitive method for structural proteomics, allowing practitioners to gain insight into protein self-assembly, including stoichiometry and three-dimensional architecture, as well as complementary thermodynamic and kinetic aspects. Although MS is typically performed in vacuum, a body of literature has described how native solution-state structure is largely retained on the timescale of the experiment. Native MS offers the benefi t that it requires substantially smaller quantities
of a sample than traditional structural techniques such as NMR and X-ray crystallography, and is therefore well suited to high-throughput studies. Here we fi rst describe the native MS approach and outline the structural proteomic data that it can deliver. We then provide practical details of experiments
to examine the structural and dynamic properties of protein assemblies, highlighting potential pitfalls as well as principles of best practice.

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http://www.tofms.org/doc/NMS_Proteomics[tofms.org].pdf

【wkh, 2015-03-26 11:30:32】 【责任人 wkh】 [已阅读 1034 次]